• Members 2331 posts
    March 4, 2024, 4:31 a.m.

    but thats the whole point my microscope objectives are eq to f16 ff which is equal to f45 m43. all the microscope sites say the same. large pixels are better ,theres no argument unless you want to post some of your 10x shots to prove me wrong 😜

  • Members 216 posts
    March 4, 2024, 4:44 a.m.

    First you have to understand why FF f16 is equal to f45 on m43? here is a hint it has nothing to do with the pixel size

  • Members 2331 posts
    March 4, 2024, 4:52 a.m.

    so are you saying that the om1 26 meg sensor has the same diffraction limit to the gh5s 10 meg 🤔

  • Members 216 posts
    March 4, 2024, 4:55 a.m.

    What is causing the diffraction is it the sensor or is it the lens and how much it has been stopped down ?
    If it is the lens then why would the sensor behind it cause more diffraction?

  • Members 2331 posts
    March 4, 2024, 4:57 a.m.

    last word on the subject

    “Diffraction is related to pixel size, not sensor size. The smaller the pixels the sooner diffraction effects will be noticed.

    blog.kasson.com/the-last-word/diffraction-and-sensors/#:~:text=%E2%80%9CDiffraction%20is%20related%20to%20pixel,of%20the%20same%20sensor%20dimensions.%E2%80%9D

  • Members 216 posts
    March 4, 2024, 5:03 a.m.

    Do you even read what you link to
    blog.kasson.com/the-last-word/diffraction-and-sensors/#
    "The first conflation is the lack of distinction between the effects of diffraction and the visibility of those effects. The size of the Airy disk is not a function of sensor size, pixel pitch, or pixel aperture. The size of the Airy disk on the sensor is a function of wavelength and f-stop. That’s all. The size of the Airy disk on the print is a function of both those, plus the ratio of sensor size to print size."

  • Members 2331 posts
    March 4, 2024, 5:29 a.m.

    edmond optics site

    f4 to 5um

    Screenshot 2024-03-04 152741.jpg

    Screenshot 2024-03-04 152741.jpg

    JPG, 106.9 KB, uploaded by DonaldB on March 4, 2024.

  • Members 2331 posts
    March 4, 2024, 5:35 a.m.

    this is my tax guide.

    my pixels are 5um my objectives are f4 I have balenced my books 🤨😎😜

    Screenshot 2024-03-04 152741.jpg

    www.edmundoptics.es/knowledge-center/application-notes/imaging/limitations-on-resolution-and-contrast-the-airy-disk/#:~:text=Every%20lens%20has%20an%20upper,will%20still%20be%20diffraction%20limited.

    Screenshot 2024-03-04 152741.jpg

    JPG, 106.9 KB, uploaded by DonaldB on March 4, 2024.

  • Members 2331 posts
    March 4, 2024, 5:59 a.m.

    Some eye candy while were at it, just shot this image. sony a74 crop mode 12meg image. just for the broken record this blows away anything ive shot with useing 20meg m43 sensor. 130 image stack using zerene. shot through 0.17 mm glass cover slip glass. 4x amscope objective so efectivly 6x

    2024-03-04-03.04.22 ZS PMax copyfs.jpg

    2024-03-04-03.04.22 ZS PMax copyfs.jpg

    JPG, 8.4 MB, uploaded by DonaldB on March 4, 2024.

  • Removed user
    March 4, 2024, 6:20 a.m.
  • Members 2331 posts
    March 4, 2024, 6:31 a.m.
  • Members 2331 posts
    March 4, 2024, 6:37 a.m.

    microscopecentral.com/pages/choosing-a-microscope-camera-what-to-look-for

    but i would read the whole page. 😁

    Quote: Although it seems counter-intuitive, higher magnification objectives actually require fewer pixels. In fact, if you are working at a higher magnification, the optical system is limited to about 3–5 Megapixels that can be transferred to the sensor of a camera. Therefore, if you go out and purchase that 20 Megapixel camera hoping to maximize clarity, know that the “extra pixels” will have NO EFFECT on the resolution of the image, while negatively impacting speed, capacity and sensitivity. However, for lower magnification, a higher pixel count will capture more detail from your microscope.

  • Members 166 posts
    March 4, 2024, 6:52 a.m.

    Having a controlled environment is not the same as controlling all the variables in a testing methodology. You clearly did not eliminate extraneous variables so that only pixel size varied. The exposures look different, the focus points look inconsistent, and you introduced variables associated with image stacking.

  • Members 1173 posts
    March 4, 2024, 7:56 a.m.

    I thought it was the other way around...

  • Members 2331 posts
    March 4, 2024, 7:57 a.m.

    yeh. i have no idea what im doing 🙄

  • Members 1173 posts
    March 4, 2024, 8 a.m.

    Where did you get the idea that the om1 is 26MP sensor, and where did you get the idea that the GH5 is 10MP sensor???

    They are both 20MP...

  • Members 2331 posts
    March 4, 2024, 8:03 a.m.

    The Panasonic GH5S is a video-focused Micro Four Thirds camera built around what the company markets as a 10.2MP sensor.

    gh6 26 meg, been a while

  • Members 1173 posts
    March 4, 2024, 8:33 a.m.

    Ok I wasn't aware of the S version. But why even mention a "video centric" version in this discussion???